| Course ID: | PBS-SE>DIAINV |
| Course title: | MOLECULAR DIAGNOSTIC AND IN VITRO PLANT TISSUE IN ECOSYSTEM AND AGROECOSYSTEM |
| Semester: | 5 / Winter |
| ECTS: | 3 |
| Lectures/Classes: | 15 / 30 hours |
| Field of study: | Applied Plant Biotechnology |
| Study cycle: | 1st cycle |
| Type of course: | compulsory |
| Prerequisites: | Knowledge of classical and molecular genetics, knowledge of the genome structure of eukaryotic organisms, knowledge of DNA organization in eukaryotic organisms, types of DNA sequences. Knowledge of basic molecular techniques such as PCR and electrophoresis. Knowledge of English at communicative level. Knowledge of plant anatomy. |
| Contact person: | Renata Galek renata.galek@upwr.edu.pl |
| Short description: | This course aims to familiarize students with genotyping techniques with DNA markers. Genotyping techniques such as RAPD, RFLP, AFLP, and SNP are most commonly used in plant biotechnology used to evaluate gene resources in ecosystems and agro-ecosystems. In addition, the subject will expand knowledge of plant-based cultures in vitro in the context of preserving gene resources and the production of pathogen-free plants. |
| Full description: | Student will learn basic plant genotyping techniques such as RAPD, RFLP, AFLP, and SNP. Topics related to the use of molecular markers will be discussed, depending on the group of plants or populations that inhabit the ecosystem and its uses (genetic diversity, identity, population erosion, mutation identification). In addition, students will be taught the practical and researched use of various techniques in plant tissue cultures. Particular attention will be paid to the benefits of using these methods in plant breeding (simplification and acceleration of biotic and abiotic stresses and mass production of healthy plant material.) Knowing how to obtain in vitro new varieties by induction and spontaneous mutation, protoplast fusion, The mechanisms of somatic embryogenesis and the use of in vitro techniques for the storage of germplasm - under conditions of slow growth and cryopreservation are also presented. |
| Bibliography: | Diagnostics in Plant Breeding, Lübberstedt,Thomas, Varshney, Rajeev 2013 Springer |
| Learning outcomes: | Knowledge Students are knowledgeable about the use of biotechnological methods in sustainable agricultural production and the conservation of nature, including the identification of genetic erosion, mutation. Students know the principles of selection of molecular diagnostic techniques based on the goal set, including the assessment of the state of genetic resources of ecosystems and agro-ecosystems. Skills Man have a working knowledge of conducting PCR Students knows the rules for the preparation and electrophoresis analysis of the electrophoretic separation of nucleic acids Social competentes Can work in a group, taking on different roles, he is responsible for own work and teamwork Identifies priorities for implementation posed by yourself or other tasks Defines the priorities for accomplishing the tasks he / she or his /her task |
| Assessment methods and assessment criteria: | Assessment of attitudes in lectures 25%, control work 50% and reports of exercises 25% |